Hotspot

psbA-trnK-UUU

Genome · 1063-1358 · 296 bp

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Pi 0.2
MarkerSeek score 63.10
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
Genome
length_bp
296
variable_sites
1
pi
0.2
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.02
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 GTGCTAGTGATCCAATCGCA GAAAGCCGTGTGCAATGAAA 50.0 45.0 58.1 57.9 487 bp 1.0 82.6
psbA-trnK-UUU_p2 GTGCTAGTGATCCAATCGCA CACGGTTTGGGGAGAGATTT 50.0 50.0 58.1 57.8 459 bp 1.0 82.1
psbA-trnK-UUU_p3 AGTGCTAGTGATCCAATCGC GAAAGCCGTGTGCAATGAAA 50.0 45.0 57.8 57.9 488 bp 1.0 81.3
psbA-trnK-UUU_p4 ATTCCATAGGCTTGCGCTTT GAAAGCCGTGTGCAATGAAA 45.0 45.0 58.2 57.9 461 bp 1.0 81.3
psbA-trnK-UUU_p5 AGTGCTAGTGATCCAATCGC CACGGTTTGGGGAGAGATTT 50.0 50.0 57.8 57.8 460 bp 1.0 80.7