primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	GTGCTAGTGATCCAATCGCA	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	58.1	57.9	3.6	0.0	0.192766	1063	1358	487-487	1.000000	82.6
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	GTGCTAGTGATCCAATCGCA	CACGGTTTGGGGAGAGATTT	20	20	50.0	50.0	58.1	57.8	3.6	0.0	0.260398	1063	1358	459-459	1.000000	82.1
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	AGTGCTAGTGATCCAATCGC	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.363955	1063	1358	488-488	1.000000	81.3
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	ATTCCATAGGCTTGCGCTTT	GAAAGCCGTGTGCAATGAAA	20	20	45.0	45.0	58.2	57.9	9.7	0.0	0.364981	1063	1358	461-461	1.000000	81.3
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	AGTGCTAGTGATCCAATCGC	CACGGTTTGGGGAGAGATTT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.431587	1063	1358	460-460	1.000000	80.7
trnK-UUU_p1	trnK-UUU	1	CCGAATAGTGGGATGCTACC	AGGAATCTTGTTGAACGGCT	20	20	55.0	45.0	57.5	57.4	0.0	0.0	1.025989	1359	3876	2994-2996	1.000000	77.1
trnK-UUU_p2	trnK-UUU	2	AGTGGGATGCTACCTCTCTT	AGGAATCTTGTTGAACGGCT	20	20	50.0	45.0	57.4	57.4	0.0	0.0	1.128004	1359	3876	2988-2990	1.000000	76.3
trnK-UUU_p3	trnK-UUU	3	GTGGGATGCTACCTCTCTTG	AGGAATCTTGTTGAACGGCT	20	20	55.0	45.0	57.4	57.4	0.0	0.0	1.179848	1359	3876	2987-2989	1.000000	75.9
trnK-UUU_p4	trnK-UUU	4	CCGAATAGTGGGATGCTACC	TCCCAACATTTCGGGATTGA	20	20	55.0	45.0	57.5	57.1	0.0	0.0	1.346536	1359	3876	2908-2910	1.000000	74.6
trnK-UUU_p5	trnK-UUU	5	CCGAATAGTGGGATGCTACC	CCCAACATTTCGGGATTGAC	20	20	55.0	50.0	57.5	57.1	0.0	0.0	1.396826	1359	3876	2907-2909	1.000000	74.2
matK_p1	matK	1	TGATCCTGGCAATTGCTCAA	GAGGTTATCCGGGGGTTTTC	20	20	45.0	55.0	57.8	58.0	28.4	0.0	0.267525	1596	3113	1591-1591	1.000000	83.8
matK_p2	matK	2	TTGATCCTGGCAATTGCTCA	GAGGTTATCCGGGGGTTTTC	20	20	45.0	55.0	57.8	58.0	28.4	0.0	0.267525	1596	3113	1592-1592	1.000000	83.8
matK_p3	matK	3	ATTTGGTTGGATTTCCGGGT	GAGGTTATCCGGGGGTTTTC	20	20	45.0	55.0	57.7	58.0	0.0	0.0	0.367469	1596	3113	1636-1636	1.000000	82.9
matK_p4	matK	4	TGATCCTGGCAATTGCTCAA	GGTTATCCGGGGGTTTTCAA	20	20	45.0	50.0	57.8	57.8	28.4	0.0	0.438161	1596	3113	1589-1589	1.000000	82.4
matK_p5	matK	5	TTGATCCTGGCAATTGCTCA	GGTTATCCGGGGGTTTTCAA	20	20	45.0	50.0	57.8	57.8	28.4	0.0	0.438161	1596	3113	1590-1590	1.000000	82.4
trnK-UUU-chlB_p1	trnK-UUU-chlB	1	AACGACATGCTGCTTTTTCC	CTCACATCGAACCATCTGCA	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.436611	3877	5892	2606-2606	0.333333	49.8
trnK-UUU-chlB_p2	trnK-UUU-chlB	2	AACGACATGCTGCTTTTTCC	CGAGAAATGGGGATTCGTGT	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.446451	3877	5892	2742-2742	0.333333	49.8
trnK-UUU-chlB_p3	trnK-UUU-chlB	3	TGAGGAACAGAAGCTCGTTC	CTCACATCGAACCATCTGCA	20	20	50.0	50.0	57.6	58.0	12.8	0.0	0.454875	3877	5892	2858-2858	0.333333	49.7
trnK-UUU-chlB_p4	trnK-UUU-chlB	4	GATCGCTCTCCTGACTCATG	TTTGCTCTGGGTATGGAGGA	20	20	55.0	50.0	57.6	58.0	0.0	0.0	0.455214	3877	5892	2868-2868	0.333333	49.7
trnK-UUU-chlB_p5	trnK-UUU-chlB	5	AACGACATGCTGCTTTTTCC	TTTGCTCTGGGTATGGAGGA	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.456862	3877	5892	2427-2427	0.333333	49.7
atpI-atpH_p1	atpI-atpH	1	CGAATCCATGGAGGGTCATC	CCTACGGATAATCCAGCAGC	20	20	55.0	55.0	57.8	57.9	21.3	0.0	0.261932	37635	38230	674-759	1.000000	79.5
atpI-atpH_p2	atpI-atpH	2	CGAATCCATGGAGGGTCATC	GCCTACGGATAATCCAGCAG	20	20	55.0	55.0	57.8	57.9	21.3	0.0	0.261932	37635	38230	675-760	1.000000	79.5
atpI-atpH_p3	atpI-atpH	3	CGAATCCATGGAGGGTCATC	AACGGAAGCAGCAGAAATCA	20	20	55.0	45.0	57.8	58.1	21.3	0.0	0.279658	37635	38230	651-736	1.000000	79.5
atpI-atpH_p4	atpI-atpH	4	CGAATCCATGGAGGGTCATC	ACGGAAGCAGCAGAAATCAA	20	20	55.0	45.0	57.8	58.1	21.3	0.0	0.279658	37635	38230	650-735	1.000000	79.5
atpI-atpH_p5	atpI-atpH	5	CGAATCCATGGAGGGTCATC	GCCAGGTCCAATAGAAGCAA	20	20	55.0	50.0	57.8	57.9	21.3	0.0	0.304648	37635	38230	696-781	1.000000	79.2
rrn5-rrn4.5_p1	rrn5-rrn4.5	1	CGAGATGGATTGGTGTGGTT	CCACCTCAAGATGAGTGCTC	20	20	50.0	55.0	57.9	58.0	0.0	6.6	0.135216	59338	59656	483-657	1.000000	81.3
rrn5-rrn4.5_p2	rrn5-rrn4.5	2	GATTGGTGTGGTTCCCCTAC	CCACCTCAAGATGAGTGCTC	20	20	55.0	55.0	57.9	58.0	0.0	6.6	0.145632	59338	59656	476-650	1.000000	81.2
rrn5-rrn4.5_p3	rrn5-rrn4.5	3	CGAGATGGATTGGTGTGGTT	AGACGAGCCGTTAGGTGATA	20	20	50.0	50.0	57.9	58.0	0.0	1.8	0.162304	59338	59656	448-622	1.000000	81.2
rrn5-rrn4.5_p4	rrn5-rrn4.5	4	GATTGGTGTGGTTCCCCTAC	AGACGAGCCGTTAGGTGATA	20	20	55.0	50.0	57.9	58.0	0.0	1.8	0.172721	59338	59656	441-615	1.000000	81.2
rrn5-rrn4.5_p5	rrn5-rrn4.5	5	AACCACCAAGTTCGAGATGG	CCACCTCAAGATGAGTGCTC	20	20	50.0	55.0	57.8	58.0	10.6	6.6	0.206359	59338	59656	495-669	1.000000	80.7
rpl22_p1	rpl22	1	AAAAAGAGAGAGAAACGATAAAA	GCAAGTAGTAGTATATTTGCGGC	23	23	26.1	43.5	52.4	57.7	0.0	0.0	11.901826	83653	84114	621-639	1.000000	42.2
rpl22_p2	rpl22	2	AAAAGAGAGAGAAACGATAAAAA	GCAAGTAGTAGTATATTTGCGGC	23	23	26.1	43.5	52.4	57.7	0.0	0.0	11.901826	83653	84114	620-638	1.000000	42.2
rpl22_p3	rpl22	3	AAAAAGAGAGAGAAACGATAAAAA	GCAAGTAGTAGTATATTTGCGGC	24	23	25.0	43.5	53.2	57.7	0.0	0.0	12.073429	83653	84114	621-639	1.000000	42.2
rpl22_p4	rpl22	4	AAAAAGAGAGAGAAACGATAAAA	ATTTGCGGCATAAATGGATT	23	20	26.1	35.0	52.4	54.2	0.0	2.3	12.391642	83653	84114	607-625	1.000000	42.2
rpl22_p5	rpl22	5	AAAAGAGAGAGAAACGATAAAAA	ATTTGCGGCATAAATGGATT	23	20	26.1	35.0	52.4	54.2	0.0	2.3	12.391642	83653	84114	606-624	1.000000	42.2
psbN-psbT_p1	psbN-psbT	1	CCTTTTGAAGAGCACGAGGA	CCCCCAAAGTTCCCGATAAA	20	20	50.0	50.0	57.8	57.5	0.0	0.0	0.687830	93343	93427	134-141	1.000000	80.2
psbN-psbT_p2	psbN-psbT	2	CCTTTTGAAGAGCACGAGGA	ATTTTCTTCCGAGAACCCCC	20	20	50.0	50.0	57.8	57.5	0.0	0.0	0.678999	93343	93427	150-157	1.000000	79.8
psbN-psbT_p3	psbN-psbT	3	TCCTTTTGAAGAGCACGAGG	ATTTTCTTCCGAGAACCCCC	20	20	50.0	50.0	57.8	57.5	0.0	0.0	0.678999	93343	93427	151-158	1.000000	79.8
psbN-psbT_p4	psbN-psbT	4	CGGTTATGCCCTATACACCG	CCCCCAAAGTTCCCGATAAA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.592545	93343	93427	186-193	1.000000	79.5
psbN-psbT_p5	psbN-psbT	5	CGGTTATGCCCTATACACCG	ATTTTCTTCCGAGAACCCCC	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.583713	93343	93427	202-209	1.000000	79.2
atpE-rbcL_p1	atpE-rbcL	1	AAGAGACAAGCAATCGAGGC	GCTTTGAATCCGACACTTGC	20	20	50.0	50.0	58.3	58.0	3.8	0.0	0.299177	110659	111105	554-594	1.000000	81.4
atpE-rbcL_p2	atpE-rbcL	2	GACAAGCAATCGAGGCTGAT	GCTTTGAATCCGACACTTGC	20	20	50.0	50.0	58.3	58.0	14.3	0.0	0.369556	110659	111105	550-590	1.000000	80.8
atpE-rbcL_p3	atpE-rbcL	3	GCTAGAACACGACTAGAGGC	GCTTTGAATCCGACACTTGC	20	20	55.0	50.0	57.5	58.0	0.0	0.0	0.509444	110659	111105	515-555	1.000000	80.0
atpE-rbcL_p4	atpE-rbcL	4	GGCTGATGTGGCTCTCAAAA	GCTTTGAATCCGACACTTGC	20	20	50.0	50.0	58.5	58.0	0.0	0.0	0.492849	110659	111105	537-577	1.000000	79.9
atpE-rbcL_p5	atpE-rbcL	5	AAGAGACAAGCAATCGAGGC	CGCTTTGAATCCGACACTTG	20	20	50.0	50.0	58.3	57.8	3.8	0.0	0.500519	110659	111105	555-595	1.000000	79.8
chlN-ycf1_p1	chlN-ycf1	1	TTCTCGTATGTGCTTCTGGC	AACCGCCAAGGATCCATTTT	20	20	50.0	45.0	58.0	58.1	0.0	15.8	0.072954	115358	116235	1606-2047	1.000000	84.8
chlN-ycf1_p2	chlN-ycf1	2	TTCTCGTATGTGCTTCTGGC	ACCGCCAAGGATCCATTTTT	20	20	50.0	45.0	58.0	58.1	0.0	15.8	0.072954	115358	116235	1605-2046	1.000000	84.8
chlN-ycf1_p3	chlN-ycf1	3	AGTATGGGATCGGTAGTGGG	AACCGCCAAGGATCCATTTT	20	20	55.0	45.0	58.0	58.1	0.0	15.8	0.073931	115358	116235	1679-2120	1.000000	84.6
chlN-ycf1_p4	chlN-ycf1	4	AGTATGGGATCGGTAGTGGG	ACCGCCAAGGATCCATTTTT	20	20	55.0	45.0	58.0	58.1	0.0	15.8	0.073931	115358	116235	1678-2119	1.000000	84.6
chlN-ycf1_p5	chlN-ycf1	5	TTCTCGTATGTGCTTCTGGC	TGGATGAGAAGACCACGAGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.085414	115358	116235	1639-2080	1.000000	84.6
clpP-chlN_p1	clpP-chlN	1	CATTCCTCCACCTCGACAAG	CAATCCGTCAAGGTCTCAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.031472	125480	127009	2301-2488	1.000000	87.9
clpP-chlN_p2	clpP-chlN	2	CCGCAATAGGCCCATAAACT	CAATCCGTCAAGGTCTCAGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.034479	125480	127009	2535-2722	1.000000	87.6
clpP-chlN_p3	clpP-chlN	3	GTCCGCAATAGGCCCATAAA	CAATCCGTCAAGGTCTCAGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.040076	125480	127009	2537-2724	1.000000	87.5
clpP-chlN_p4	clpP-chlN	4	TCCGCAATAGGCCCATAAAC	CAATCCGTCAAGGTCTCAGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.040076	125480	127009	2536-2723	1.000000	87.5
clpP-chlN_p5	clpP-chlN	5	ATTCCTCCACCTCGACAAGA	CAATCCGTCAAGGTCTCAGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.077151	125480	127009	2300-2487	1.000000	87.5