Hotspot

ndhJ

LSC · 49726-50177 · 452 bp

Back to Thrixspermum

Pi 0.28

MarkerSeek score 53.40

Primer available yes

Label ndhJ

Feature Metadata

label_name: ndhJ
feature_type: gene
region: LSC
length_bp: 452
variable_sites: 7
pi: 0.28
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.06
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TAGGCTTGCGATTCGACTTT	50.0	45.0	58.1	57.6	1138 bp	0.5	56.5
ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGGGAAGGTCCAGAAGAGTT	50.0	50.0	58.1	57.6	1248 bp	0.5	56.2
ndhJ_p3	GGAAATGGTCGGGATAGCTC	TAGGCTTGCGATTCGACTTT	55.0	45.0	57.8	57.6	1184 bp	0.5	55.6
ndhJ_p4	AATGGTCGGGATAGCTCAGT	TAGGCTTGCGATTCGACTTT	50.0	45.0	58.2	57.6	1181 bp	0.5	55.3
ndhJ_p5	ATGGTCGGGATAGCTCAGTT	TAGGCTTGCGATTCGACTTT	50.0	45.0	58.2	57.6	1180 bp	0.5	55.3