Hotspot

rpl32-trnL-UAG

SSC · 111989-112653 · 665 bp

Back to Thrixspermum

Pi 0.0487

MarkerSeek score 82.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 665
variable_sites: 29
pi: 0.0487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	697-764 bp	1.0	85.9
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	690-757 bp	1.0	85.4
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	696-763 bp	1.0	85.3
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	695-762 bp	1.0	85.3
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	GAGCAGCGTGTCTACCAATT	50.0	50.0	58.0	58.3	691-758 bp	1.0	84.7