Hotspot

clpP-psbB

LSC · 73194-73910 · 717 bp

Back to Thrixspermum

Pi 0.0844

MarkerSeek score 94.00

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 59
pi: 0.0844
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 94.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CAACCAGAAACCAGAGCTGT	50.0	50.0	58.0	58.0	891-1025 bp	1.0	86.2
clpP-psbB_p2	AGGCACTTTTGGAACACCAA	CAACCAGAAACCAGAGCTGT	45.0	50.0	58.1	58.0	842-976 bp	1.0	85.4
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	837-971 bp	1.0	85.3
clpP-psbB_p4	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	813-947 bp	1.0	85.1
clpP-psbB_p5	ACCCAAACTGCATCTTCCTC	CAACCAGAAACCAGAGCTGT	50.0	50.0	57.8	58.0	877-1011 bp	1.0	84.7