Hotspot

trnT-GGU-psbD

LSC · 33007-33988 · 982 bp

Back to Thrixspermum

Pi 0.0654
MarkerSeek score 79.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
982
variable_sites
61
pi
0.0654
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CTGCTACACCAAAACTGGGT 55.0 50.0 58.1 58.0 1547-1573 bp 1.0 85.0
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG GCCTAAGACTCCAGCAACTC 55.0 55.0 58.1 58.0 1636-1662 bp 1.0 84.8
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA CTGCTACACCAAAACTGGGT 50.0 50.0 58.1 58.0 1550-1576 bp 1.0 84.8
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG TAAGACTCCAGCAACTCCCA 55.0 50.0 58.1 58.0 1633-1659 bp 1.0 84.7
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA GCCTAAGACTCCAGCAACTC 50.0 55.0 58.1 58.0 1639-1665 bp 1.0 84.6