Hotspot

trnY-GUA-trnE-UUC

LSC · 32042-32100 · 59 bp

Back to Thrixspermum

Pi 0.136

MarkerSeek score 78.30

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 8
pi: 0.136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	TGGAGCGACGAATTATTGGG	TCTCTTTCAAGGAGGCAACG	50.0	50.0	58.1	57.8	203 bp	1.0	81.4
trnY-GUA-trnE-UUC_p2	GAACCAGCGTAGACATCTCG	TCTCTTTCAAGGAGGCAACG	55.0	50.0	58.2	57.8	170 bp	1.0	81.0
trnY-GUA-trnE-UUC_p3	TGGAGCGACGAATTATTGGG	TCGTCTAGTGGTTCAGGACA	50.0	50.0	58.1	57.7	225 bp	1.0	80.2
trnY-GUA-trnE-UUC_p4	CGAATTTACAGTCCGTCCCC	TCTCTTTCAAGGAGGCAACG	55.0	50.0	58.4	57.8	146 bp	1.0	80.2
trnY-GUA-trnE-UUC_p5	GAACCAGCGTAGACATCTCG	TCGTCTAGTGGTTCAGGACA	55.0	50.0	58.2	57.7	192 bp	1.0	79.7