Hotspot

psbM-trnD-GUC

LSC · 30465-31571 · 1107 bp

Back to Thrixspermum

Pi 0.0949

MarkerSeek score 91.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1107
variable_sites: 102
pi: 0.0949
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 91.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAGCGCAGTAGCAATAAACG	AGTTCAATCGGTCAGAGCAC	50.0	50.0	57.9	57.9	1212-1243 bp	1.0	87.4
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1217-1248 bp	1.0	86.0
psbM-trnD-GUC_p3	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1269-1300 bp	1.0	85.6
psbM-trnD-GUC_p4	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.6	57.9	1216-1247 bp	1.0	85.0
psbM-trnD-GUC_p5	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.5	57.9	1218-1249 bp	1.0	84.2