Hotspot

rpoB-trnC-GCA

LSC · 27352-28552 · 1201 bp

Back to Thrixspermum

Pi 0.0508

MarkerSeek score 81.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1201
variable_sites: 60
pi: 0.0508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTGGACATTCCCTCATTTCCA	CCGGGGATAAAGGATTTGCA	47.6	50.0	57.9	57.9	1284-1334 bp	1.0	75.7
rpoB-trnC-GCA_p2	TGTGGACATTCCCTCATTTCC	CCGGGGATAAAGGATTTGCA	47.6	50.0	57.9	57.9	1285-1335 bp	1.0	75.7
rpoB-trnC-GCA_p3	GTGGACATTCCCTCATTTCCA	ACCGGGGATAAAGGATTTGC	47.6	50.0	57.9	57.9	1285-1335 bp	1.0	75.7
rpoB-trnC-GCA_p4	TGTGGACATTCCCTCATTTCC	ACCGGGGATAAAGGATTTGC	47.6	50.0	57.9	57.9	1286-1336 bp	1.0	75.7
rpoB-trnC-GCA_p5	GTGGACATTCCCTCATTTCCA	TCGAACCGGGGATAAAGGAT	47.6	50.0	57.9	58.2	1289-1339 bp	1.0	74.6