Hotspot

matK-trnK-UUU

LSC · 3314-4519 · 1206 bp

Back to Thrixspermum

Pi 0.0553

MarkerSeek score 76.80

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1206
variable_sites: 64
pi: 0.0553
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCCTGAAGGAGTAGCGGATA	TGGGTTGCTAACTCAATGGT	50.0	45.0	57.0	57.0	1351-1435 bp	1.0	70.4
matK-trnK-UUU_p2	TCCTGAAGGAGTAGCGGATA	TCAATGGTAGAGTACTCGGCT	50.0	47.6	57.0	58.0	1339-1423 bp	1.0	70.1
matK-trnK-UUU_p3	TCCTGAAGGAGTAGCGGATA	TCTGGGTTGCTAACTCAATGG	50.0	47.6	57.0	57.9	1353-1437 bp	1.0	69.6
matK-trnK-UUU_p4	TCCTGAAGGAGTAGCGGATA	CTGGGTTGCTAACTCAATGGT	50.0	47.6	57.0	58.2	1352-1436 bp	1.0	68.5
matK-trnK-UUU_p5	TCCTGAAGGAGTAGCGGATA	CTCAATGGTAGAGTACTCGGC	50.0	52.4	57.0	57.6	1340-1424 bp	1.0	67.4