Hotspot

ycf1

SSC · 128887-134541 · 5655 bp

Back to Thomsonaria

Pi 0.00116

MarkerSeek score 40.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5655
variable_sites: 17
pi: 0.00116
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TATCCCAGGCTTCCGCTATT	GAAGCAAATGCACATTCCCC	50.0	50.0	58.3	58.0	244 bp	1.0	79.0
ycf1_p2	CCAGGCTTCCGCTATTTGTA	GAAGCAAATGCACATTCCCC	50.0	50.0	57.7	58.0	240 bp	1.0	78.6
ycf1_p3	TATCCCAGGCTTCCGCTATT	AAGCAAATGCACATTCCCCT	50.0	45.0	58.3	58.4	243 bp	1.0	76.3
ycf1_p4	CCAGGCTTCCGCTATTTGTA	AAGCAAATGCACATTCCCCT	50.0	45.0	57.7	58.4	239 bp	1.0	75.9
ycf1_p5	TATCCCAGGCTTCCGCTATT	CGCGCTCAAAGACGTAAAAC	50.0	50.0	58.3	58.4	283 bp	1.0	75.6