Hotspot

clpP

LSC · 73349-75381 · 2033 bp

Back to Thomsonaria

Pi 0.00228

MarkerSeek score 51.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2033
variable_sites: 12
pi: 0.00228
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCTCAAATCACCAATTCGTCTGA	ACGCTTTCACATCAAAGTGA	39.1	40.0	58.1	55.9	2121-2132 bp	1.0	40.8
clpP_p2	CTCAAATCACCAATTCGTCTGA	ACGCTTTCACATCAAAGTGA	40.9	40.0	56.7	55.9	2120-2131 bp	1.0	40.8
clpP_p3	TCTCAAATCACCAATTCGTCTG	ACGCTTTCACATCAAAGTGA	40.9	40.0	56.7	55.9	2121-2132 bp	1.0	40.8
clpP_p4	TCTCAAATCACCAATTCGTCTGA	TCCATTGTTACGCTTTCACA	39.1	40.0	58.1	55.6	2130-2141 bp	1.0	40.8
clpP_p5	TCTCAAATCACCAATTCGTCTGA	CCATTGTTACGCTTTCACATCA	39.1	40.9	58.1	57.6	2129-2140 bp	1.0	40.8