Hotspot

rpl32-trnL-UAG

SSC · 117593-119081 · 1489 bp

Back to Thomsonaria

Pi 0.0058
MarkerSeek score 54.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1489
variable_sites
18
pi
0.0058
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1518-1601 bp 1.0 83.5
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1519-1602 bp 1.0 83.5
rpl32-trnL-UAG_p3 TAGGGCAGCATTGAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1520-1603 bp 1.0 83.1
rpl32-trnL-UAG_p4 TAGGGCAGCATTGAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1521-1604 bp 1.0 83.1
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1528-1611 bp 1.0 82.5