Hotspot

rps11-rpl36

LSC · 82880-83054 · 175 bp

Back to Thomsonaria

Pi 0.00571

MarkerSeek score 50.30

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 3
pi: 0.00571
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ACGTCTATTTCTACGCGAACC	AAAATAAGGGCCTCCGTTCG	47.6	50.0	58.2	58.3	320-332 bp	1.0	71.7
rps11-rpl36_p2	CGTCTATTTCTACGCGAACCA	AAAATAAGGGCCTCCGTTCG	47.6	50.0	58.2	58.3	319-331 bp	1.0	71.7
rps11-rpl36_p3	ACGTCTATTTCTACGCGAACC	AATAAGGGCCTCCGTTCGTA	47.6	50.0	58.2	58.5	318-330 bp	1.0	69.7
rps11-rpl36_p4	ACGTCTATTTCTACGCGAACC	ATAAGGGCCTCCGTTCGTAA	47.6	50.0	58.2	58.5	317-329 bp	1.0	69.7
rps11-rpl36_p5	CGTCTATTTCTACGCGAACCA	ATAAGGGCCTCCGTTCGTAA	47.6	50.0	58.2	58.5	316-328 bp	1.0	69.7