Hotspot

psaJ-rpl33

LSC · 70475-70947 · 473 bp

Back to Thomsonaria

Pi 0.00446

MarkerSeek score 49.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 9
pi: 0.00446
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGTTGATATTCC	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	562-583 bp	1.0	73.4
psaJ-rpl33_p2	TCCGGATGCGTTGATATTCC	TGGAAATGCCCATTGATTCCT	50.0	42.9	57.8	57.9	589-610 bp	1.0	73.1
psaJ-rpl33_p3	GTTCGGGTCTTTAGCAGGTT	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	607-628 bp	1.0	73.0
psaJ-rpl33_p4	GTTCGGGTCTTTAGCAGGTT	TGGAAATGCCCATTGATTCCT	50.0	42.9	57.8	57.9	634-655 bp	1.0	72.7
psaJ-rpl33_p5	TGGCACCGGTAGTAAGTACT	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	632-653 bp	1.0	72.6