Hotspot

accD-psaI

LSC · 61873-62664 · 792 bp

Back to Thomsonaria

Pi 0.00421

MarkerSeek score 36.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 15
pi: 0.00421
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCATGCAAGAAGGAAGCTTG	GTTTTTCGAGACCCCGCTAT	50.0	50.0	58.0	58.0	1732-1891 bp	1.0	81.1
accD-psaI_p2	GCATGCAAGAAGGAAGCTTG	TCGAGACCCCGCTATAAGTT	50.0	50.0	58.0	57.9	1727-1886 bp	1.0	80.8
accD-psaI_p3	GCATGCAAGAAGGAAGCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1230-1384 bp	1.0	80.7
accD-psaI_p4	GCATGCAAGAAGGAAGCTTG	AATGGAGGGGAGACTGTTGA	50.0	50.0	58.0	58.0	1187-1341 bp	1.0	80.6
accD-psaI_p5	GCATGCAAGAAGGAAGCTTG	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.0	58.0	1221-1375 bp	1.0	80.6