Hotspot

ndhC-trnV-UAC

LSC · 53925-54537 · 613 bp

Back to Thomsonaria

Pi 0.0101
MarkerSeek score 57.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
613
variable_sites
14
pi
0.0101
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT GCACGTTGGGTCTTTGAAAC 50.0 50.0 57.9 58.2 776-818 bp 1.0 83.0
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 712-754 bp 1.0 82.9
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AATGCACGTTGGGTCTTTGA 50.0 45.0 57.9 58.3 779-821 bp 1.0 81.8
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT ATGCACGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 778-820 bp 1.0 81.8
ndhC-trnV-UAC_p5 CTGGTCCTTTGCTAATCGGG GCACGTTGGGTCTTTGAAAC 55.0 50.0 58.3 58.2 793-835 bp 1.0 81.4