Hotspot

trnF-GAA-ndhJ

LSC · 51527-52221 · 695 bp

Back to Thomsonaria

Pi 0.000641

MarkerSeek score 49.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 2
pi: 0.000641
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	869-880 bp	1.0	79.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	833-844 bp	1.0	79.5
trnF-GAA-ndhJ_p3	GTCGTGAATGGTCGGGATAG	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	882-893 bp	1.0	78.9
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	857-868 bp	1.0	78.9
trnF-GAA-ndhJ_p5	GAGGATGATGCGTCGTGAAT	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	893-904 bp	1.0	78.8