Hotspot

trnR-UCU-atpA

LSC · 10154-10860 · 707 bp

Back to Thomsonaria

Pi 0.0293

MarkerSeek score 83.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 59
pi: 0.0293
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	792-939 bp	1.0	73.8
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	791-938 bp	1.0	73.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	731-878 bp	1.0	73.3
trnR-UCU-atpA_p4	TGGAATGAAAAGCGTCCATT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	792-939 bp	1.0	69.8
trnR-UCU-atpA_p5	TTGGAATGAAAAGCGTCCAT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	793-940 bp	1.0	69.8