Hotspot

rps16-trnQ-UUG

LSC · 6397-7205 · 809 bp

Back to Thomsonaria

Pi 0.0024
MarkerSeek score 59.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
809
variable_sites
5
pi
0.0024
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 877-897 bp 1.0 80.3
rps16-trnQ-UUG_p2 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 887-907 bp 1.0 78.6
rps16-trnQ-UUG_p3 GCTTTCTGCCACATCGTTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 854-874 bp 1.0 78.6
rps16-trnQ-UUG_p4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 888-908 bp 1.0 78.5
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GTGTCGACTAGAAATGGGGC 50.0 55.0 58.2 58.3 939-959 bp 1.0 77.9