Hotspot

ycf1

SSC · 123300-128627 · 5328 bp

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Pi 0.0626

MarkerSeek score 68.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5328
variable_sites: 331
pi: 0.0626
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCATCGCAATTGAAAACGC	TTACCTCAGATTGCATGGGC	45.0	50.0	58.0	57.9	940-985 bp	1.0	86.2
ycf1_p2	CCGCTTCGGTATTGCTTTTG	TTACCTCAGATTGCATGGGC	50.0	50.0	58.1	57.9	892-937 bp	1.0	85.9
ycf1_p3	TCCATCGCAATTGAAAACGC	CCTCAGATTGCATGGGCTAG	45.0	55.0	58.0	58.1	937-982 bp	1.0	85.8
ycf1_p4	CTTGACCTGCCGAGTCTTTT	TTACCTCAGATTGCATGGGC	50.0	50.0	58.1	57.9	778 bp	0.5	58.6
ycf1_p5	TGACCTGCCGAGTCTTTTTC	TTACCTCAGATTGCATGGGC	50.0	50.0	58.1	57.9	776 bp	0.5	58.6