Hotspot

rpl32-trnL-TAG

SSC · 113054-113728 · 675 bp

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Pi 0.128
MarkerSeek score 80.80
Primer available yes
Label rpl32-trnL-TAG

Feature Metadata

label_name
rpl32-trnL-TAG
feature_type
igs
region
SSC
length_bp
675
variable_sites
72
pi
0.128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-TAG_p1 TCCAGTACACTAAGCGAGCT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 1221-1389 bp 1.0 87.4
rpl32-trnL-TAG_p2 TCCAGTACACTAAGCGAGCT AGCAGCGTGTCTACCAATTT 50.0 45.0 58.2 57.8 1214-1382 bp 1.0 86.9
rpl32-trnL-TAG_p3 TCCAGTACACTAAGCGAGCT CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.2 57.8 1220-1388 bp 1.0 86.8
rpl32-trnL-TAG_p4 TCCAGTACACTAAGCGAGCT CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.2 57.8 1219-1387 bp 1.0 86.8
rpl32-trnL-TAG_p5 GGAAACGGAAGGGGTATTGG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.2 57.9 815 bp 0.5 57.1