Hotspot

rpl16-rps3

LSC · 82045-82225 · 181 bp

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Pi 0.093

MarkerSeek score 80.40

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 16
pi: 0.093
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	GTTCGAACTATTTATGGGGTCT	45.0	40.9	57.6	55.6	258-267 bp	1.0	49.4
rpl16-rps3_p2	AAACCAACGAGTCACACACT	CGAACTATTTATGGGGTCTTAGGA	45.0	41.7	57.6	57.6	255-264 bp	1.0	49.2
rpl16-rps3_p3	AAACCAACGAGTCACACACT	TCGAACTATTTATGGGGTCTTAGG	45.0	41.7	57.6	57.6	256-265 bp	1.0	49.2
rpl16-rps3_p4	AAACCAACGAGTCACACACT	CGAACTATTTATGGGGTCTTAGG	45.0	43.5	57.6	56.2	255-264 bp	1.0	47.7
rpl16-rps3_p5	AAACCAACGAGTCACACACT	GTTCGAACTATTTATGGGGTCTT	45.0	39.1	57.6	56.3	258-267 bp	1.0	47.6