Hotspot

psbE-petL

LSC · 64165-65030 · 866 bp

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Pi 0.263

MarkerSeek score 80.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 866
variable_sites: 182
pi: 0.263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AAAGGATCAAAACGGCCTGT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.0	58.0	1125 bp	0.5	60.0
psbE-petL_p2	AAGGATCAAAACGGCCTGTT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.0	58.0	1124 bp	0.5	60.0
psbE-petL_p3	AAAGGATCAAAACGGCCTGT	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.0	58.0	1127 bp	0.5	59.9
psbE-petL_p4	AAGGATCAAAACGGCCTGTT	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.0	58.0	1126 bp	0.5	59.9
psbE-petL_p5	ACCAGCCCGCAATAAATAGG	GCCGCTAGTAGAAAACCGAA	50.0	50.0	58.0	58.0	1013 bp	0.5	59.8