Hotspot

petA-psbJ

LSC · 62408-63379 · 972 bp

Back to Thlaspi

Pi 0.105

MarkerSeek score 70.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 972
variable_sites: 63
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTTTCAGAGGGCGAATCCAT	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1427-1776 bp	1.0	87.2
petA-psbJ_p2	GTTTCAGAGGGCGAATCCAT	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1426-1775 bp	1.0	87.2
petA-psbJ_p3	GTTTCAGAGGGCGAATCCAT	CCTATTTATTGCGGGCTGGT	50.0	50.0	58.0	58.0	1538-1887 bp	1.0	87.0
petA-psbJ_p4	AAGGGAGCTTTGAATGTGGG	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.1	58.0	1874-2223 bp	1.0	86.6
petA-psbJ_p5	AAGGGAGCTTTGAATGTGGG	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.1	58.0	1873-2222 bp	1.0	86.6