Hotspot

rbcL-accD

LSC · 56138-56847 · 710 bp

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Pi 0.0761

MarkerSeek score 80.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 51
pi: 0.0761
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TCATGGTATGCACTTCCGTG	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.0	57.9	1291-1296 bp	1.0	86.5
rbcL-accD_p2	TGCAATGCACGCTGTTATTG	TAGCTCGCCTCTGTATTCCA	45.0	50.0	58.0	57.9	1324-1329 bp	1.0	86.4
rbcL-accD_p3	TTGAATGCTACTGCGGGTAC	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.0	57.9	1491-1496 bp	1.0	85.8
rbcL-accD_p4	TTGAATGCTACTGCGGGTAC	TAACTAGAACGCTCACCCCA	50.0	50.0	58.0	58.1	1606 bp	0.5	59.3
rbcL-accD_p5	TGCAATGCACGCTGTTATTG	TAACTAGAACGCTCACCCCA	45.0	50.0	58.0	58.1	1439 bp	0.5	59.3