Hotspot

ndhC-trnV-TAC

LSC · 50074-50926 · 853 bp

Back to Thlaspi

Pi 0.0857

MarkerSeek score 77.30

Primer available yes

Label ndhC-trnV-TAC

Feature Metadata

label_name: ndhC-trnV-TAC
feature_type: igs
region: LSC
length_bp: 853
variable_sites: 57
pi: 0.0857
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-TAC_p1	GCCCAGAAAATATCATATTCGT	GTTCGAGTCCGTATAGCCCT	36.4	55.0	54.4	58.7	728-909 bp	1.0	49.6
ndhC-trnV-TAC_p2	GCCCAGAAAATATCATATTCGT	TTCGAGTCCGTATAGCCCTA	36.4	50.0	54.4	57.1	727-908 bp	1.0	49.6
ndhC-trnV-TAC_p3	GCCCAGAAAATATCATATTCGT	AGGTCTACGGTTCGAGTCC	36.4	57.9	54.4	58.1	737-918 bp	1.0	49.5
ndhC-trnV-TAC_p4	GCCCAGAAAATATCATATTCGT	TCGAACTCTTTTACCGAGCAG	36.4	47.6	54.4	58.1	756-937 bp	1.0	49.4
ndhC-trnV-TAC_p5	GCCCAGAAAATATCATATTCGT	TCGAACTCTTTTACCGAGCA	36.4	45.0	54.4	56.9	756-937 bp	1.0	49.4