Hotspot

trnF-GAA-ndhJ

LSC · 47803-48391 · 589 bp

Back to Thlaspi

Pi 0.0716

MarkerSeek score 83.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 39
pi: 0.0716
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTGGTTTACGCGAACAAAC	CATCCACGACTGAAACGGAT	50.0	50.0	58.0	58.0	1414 bp	0.5	59.7
trnF-GAA-ndhJ_p2	GGTTTACGCGAACAAACCTG	CATCCACGACTGAAACGGAT	50.0	50.0	58.0	58.0	1411 bp	0.5	59.7
trnF-GAA-ndhJ_p3	TCCTGGTTTACGCGAACAAA	CATCCACGACTGAAACGGAT	45.0	50.0	58.0	58.0	1415 bp	0.5	59.5
trnF-GAA-ndhJ_p4	CCTGGTTTACGCGAACAAAC	ATCGTTGGGCTTCGATTACC	50.0	50.0	58.0	58.1	1736 bp	0.5	59.4
trnF-GAA-ndhJ_p5	GGTTTACGCGAACAAACCTG	ATCGTTGGGCTTCGATTACC	50.0	50.0	58.0	58.1	1733 bp	0.5	59.4