Hotspot

rps4-trnT-TGT

LSC · 45496-45904 · 409 bp

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Pi 0.0699
MarkerSeek score 79.80
Primer available yes
Label rps4-trnT-TGT

Feature Metadata

label_name
rps4-trnT-TGT
feature_type
igs
region
LSC
length_bp
409
variable_sites
27
pi
0.0699
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-TGT_p1 CCCCAGACGGCGTATTTTT CTCAGAGGTTAGAGCATCGC 52.6 55.0 58.1 57.9 498-520 bp 1.0 77.7
rps4-trnT-TGT_p2 CCCCAGACGGCGTATTTTT ATGGTCATCGGTTCGATTCC 52.6 50.0 58.1 57.8 466-488 bp 1.0 77.4
rps4-trnT-TGT_p3 CCCCAGACGGCGTATTTTT CCGCTTAGCTCAGAGGTTAG 52.6 55.0 58.1 57.5 506-528 bp 1.0 74.6
rps4-trnT-TGT_p4 CCCCAGACGGCGTATTTTT CAGAGGTTAGAGCATCGCAT 52.6 50.0 58.1 57.5 496-518 bp 1.0 74.6
rps4-trnT-TGT_p5 CCCCAGACGGCGTATTTTT TCAGAGGTTAGAGCATCGCA 52.6 50.0 58.1 58.5 497-519 bp 1.0 74.6