Hotspot

trnS-TGA-psbZ

LSC · 35120-35466 · 347 bp

Back to Thlaspi

Pi 0.0636

MarkerSeek score 80.70

Primer available yes

Label trnS-TGA-psbZ

Feature Metadata

label_name: trnS-TGA-psbZ
feature_type: igs
region: LSC
length_bp: 347
variable_sites: 21
pi: 0.0636
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-TGA-psbZ_p1	TTTTCAAGACCGGAGCCATC	ACGCAAATACAACGGGTACA	50.0	45.0	58.2	57.8	460-472 bp	1.0	83.8
trnS-TGA-psbZ_p2	TTTTCAAGACCGGAGCCATC	TACTCGACCAACCATCAGGA	50.0	50.0	58.2	57.8	481-493 bp	1.0	83.0
trnS-TGA-psbZ_p3	TTTTCAAGACCGGAGCCATC	AGGAGACGCAAATACAACGG	50.0	50.0	58.2	58.3	465-477 bp	1.0	82.8
trnS-TGA-psbZ_p4	GTTTTCAAGACCGGAGCCAT	ACGCAAATACAACGGGTACA	50.0	45.0	58.5	57.8	461-473 bp	1.0	81.5
trnS-TGA-psbZ_p5	TTTTCAAGACCGGAGCCATC	CAACCATCAGGAGACGCAAA	50.0	50.0	58.2	58.5	473-485 bp	1.0	81.1