Hotspot

trnY-GTA-trnE-TTC

LSC · 30291-30349 · 59 bp

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Pi 0.119
MarkerSeek score 80.10
Primer available yes
Label trnY-GTA-trnE-TTC

Feature Metadata

label_name
trnY-GTA-trnE-TTC
feature_type
igs
region
LSC
length_bp
59
variable_sites
7
pi
0.119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GTA-trnE-TTC_p1 CCAGCGTAGACATATTGCCA AAGTATGCCCCCATCGTCTA 50.0 50.0 57.8 57.9 202 bp 1.0 80.1
trnY-GTA-trnE-TTC_p2 ACCAGCGTAGACATATTGCC AAGTATGCCCCCATCGTCTA 50.0 50.0 57.8 57.9 203 bp 1.0 80.1
trnY-GTA-trnE-TTC_p3 CGAATTTACAGTCCGTCCCC AAGTATGCCCCCATCGTCTA 55.0 50.0 58.4 57.9 181 bp 1.0 79.5
trnY-GTA-trnE-TTC_p4 ACGAATTTACAGTCCGTCCC AAGTATGCCCCCATCGTCTA 50.0 50.0 57.6 57.9 182 bp 1.0 79.3
trnY-GTA-trnE-TTC_p5 AGACAGCGAATTATTGGGCC AAGTATGCCCCCATCGTCTA 50.0 50.0 58.3 57.9 236 bp 1.0 79.0