Hotspot

trnK-TTT-rps16

LSC · 4276-4941 · 666 bp

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Pi 0.131
MarkerSeek score 85.80
Primer available yes
Label trnK-TTT-rps16

Feature Metadata

label_name
trnK-TTT-rps16
feature_type
igs
region
LSC
length_bp
666
variable_sites
80
pi
0.131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-TTT-rps16_p1 AACTCTACCGCAGATCTGGA ACGGAATTAAGTCTTAATAAAACGA 50.0 28.0 57.9 55.1 794-804 bp 1.0 52.0
trnK-TTT-rps16_p2 AAAGCCGAGTACTCTACCGT ACGGAATTAAGTCTTAATAAAACGA 50.0 28.0 58.2 55.1 726-736 bp 1.0 52.0
trnK-TTT-rps16_p3 AAGCCGAGTACTCTACCGTT ACGGAATTAAGTCTTAATAAAACGA 50.0 28.0 58.2 55.1 725-735 bp 1.0 52.0
trnK-TTT-rps16_p4 TACCGTTGAGTTAGCAACCC ACGGAATTAAGTCTTAATAAAACGA 50.0 28.0 57.5 55.1 712-722 bp 1.0 52.0
trnK-TTT-rps16_p5 AACTCTACCGCAGATCTGGA ACGGAATTAAGTCTTAATAAAACGAA 50.0 26.9 57.9 55.8 794-804 bp 1.0 52.0