Hotspot

ndhH

SSC · 124831-126012 · 1182 bp

Back to Thladiantha

Pi 0.246

MarkerSeek score 77.50

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 436
pi: 0.246
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AGGTCTCAACGAATCACACG	TGGGAAAACGCCAACGATTA	50.0	45.0	57.9	58.1	1963 bp	0.5	59.0
ndhH_p2	GGAGCAACACTTGAAATGGC	TGGGAAAACGCCAACGATTA	50.0	45.0	57.9	58.1	1816 bp	0.5	59.0
ndhH_p3	TAAGAAGTCCGATGGGAGCA	TGGGAAAACGCCAACGATTA	50.0	45.0	58.1	58.1	1830 bp	0.5	58.4
ndhH_p4	AGGTCTCAACGAATCACACG	GGGAAAACGCCAACGATTAC	50.0	50.0	57.9	57.7	1962 bp	0.5	57.8
ndhH_p5	GGAGCAACACTTGAAATGGC	GGGAAAACGCCAACGATTAC	50.0	50.0	57.9	57.7	1815 bp	0.5	57.8