Hotspot

ndhF

SSC · 112930-115176 · 2247 bp

Back to Thladiantha

Pi 0.271

MarkerSeek score 80.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 913
pi: 0.271
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	TGGATCAACTTGTAGGCTGTC	50.0	47.6	58.0	57.7	2989 bp	0.25	39.0
ndhF_p2	AAGAGGGATCCACCGAAGAA	TGGATCAACTTGTAGGCTGTC	50.0	47.6	58.0	57.7	2981 bp	0.25	38.9
ndhF_p3	ACAGGAACAAGAGGGATCCA	ACTGGATCAACTTGTAGGCTG	50.0	47.6	58.0	57.7	2991 bp	0.25	38.9
ndhF_p4	ACAGGAACAAGAGGGATCCA	CTGGATCAACTTGTAGGCTGT	50.0	47.6	58.0	57.7	2990 bp	0.25	38.9
ndhF_p5	AAGAGGGATCCACCGAAGAA	CTGGATCAACTTGTAGGCTGT	50.0	47.6	58.0	57.7	2982 bp	0.25	38.9