Hotspot

ndhD

SSC · 118473-120062 · 1590 bp

Back to Thladiantha

Pi 0.258

MarkerSeek score 79.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1590
variable_sites: 618
pi: 0.258
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCCTGACGAACACAAACATG	AAACAACTCGAAGCATGGGT	50.0	45.0	58.0	58.0	1918-1932 bp	0.5	61.0
ndhD_p2	GCCTGACGAACACAAACATG	GGCATGAAACAACTCGAAGC	50.0	50.0	58.0	58.0	1924-1938 bp	0.5	60.9
ndhD_p3	GCCTGACGAACACAAACATG	CCGACGGATTTCTTGAGTGT	50.0	50.0	58.0	57.9	1958-1972 bp	0.5	60.5
ndhD_p4	GAATTCACGAAAGGGCCTGA	AAACAACTCGAAGCATGGGT	50.0	45.0	58.2	58.0	1932-1946 bp	0.5	59.9
ndhD_p5	GCCTGACGAACACAAACATG	TTCGGCTCCAAGAACAGAAG	50.0	50.0	58.0	57.8	2013 bp	0.25	48.3