Hotspot

ndhA

SSC · 122608-124829 · 2222 bp

Back to Thladiantha

Pi 0.285

MarkerSeek score 81.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2222
variable_sites: 953
pi: 0.285
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGCAGCTCGTACTGTTTGTT	CAGACTTGGTGAGATGACGG	45.0	55.0	58.1	58.0	2754-2757 bp	0.5	60.1
ndhA_p2	TTGCAGCTCGTACTGTTTGT	CAGACTTGGTGAGATGACGG	45.0	55.0	58.1	58.0	2755-2758 bp	0.5	60.1
ndhA_p3	TGCAGCTCGTACTGTTTGTT	ATTGGGGGTTATCAGGACCA	45.0	50.0	58.1	58.0	2896-2899 bp	0.5	60.1
ndhA_p4	TGCAGCTCGTACTGTTTGTT	TGGGGGTTATCAGGACCAAT	45.0	50.0	58.1	58.0	2894-2897 bp	0.5	60.1
ndhA_p5	TTGCAGCTCGTACTGTTTGT	TGGGGGTTATCAGGACCAAT	45.0	50.0	58.1	58.0	2895-2898 bp	0.5	60.1