Hotspot

rps15-ycf1

SSC · 126390-126800 · 411 bp

Back to Thladiantha

Pi 0.292

MarkerSeek score 78.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 411
variable_sites: 179
pi: 0.292
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TAATCGTTGGCGTTTTCCCA	AAAGCTCTTTCTTTGGCCCA	45.0	45.0	58.1	57.9	713-733 bp	0.5	62.1
rps15-ycf1_p2	TAATCGTTGGCGTTTTCCCA	AAGCTCTTTCTTTGGCCCAA	45.0	45.0	58.1	57.9	712-732 bp	0.5	62.1
rps15-ycf1_p3	ATGAGTCGAAGAACACCGTG	AAAGCTCTTTCTTTGGCCCA	50.0	45.0	57.9	57.9	987-1007 bp	0.5	61.4
rps15-ycf1_p4	ATGAGTCGAAGAACACCGTG	AAGCTCTTTCTTTGGCCCAA	50.0	45.0	57.9	57.9	986-1006 bp	0.5	61.4
rps15-ycf1_p5	CGTTTCTTGTAGCTGGTCCA	AAGCTCTTTCTTTGGCCCAA	50.0	45.0	57.8	57.9	925-945 bp	0.5	60.8