Hotspot

ndhG-ndhI

SSC · 121672-122032 · 361 bp

Back to Thladiantha

Pi 0.256

MarkerSeek score 76.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 361
variable_sites: 140
pi: 0.256
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CATGTATCGGTCCAGGCAAA	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	842-844 bp	0.5	60.5
ndhG-ndhI_p2	CATGTATCGGTCCAGGCAAA	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	841-843 bp	0.5	60.5
ndhG-ndhI_p3	CAAGAACCAGTCCCAACGAA	AACAAACAGTACGAGCTGCA	50.0	45.0	58.0	58.1	947-949 bp	0.5	60.5
ndhG-ndhI_p4	CAAGAACCAGTCCCAACGAA	ACAAACAGTACGAGCTGCAA	50.0	45.0	58.0	58.1	946-948 bp	0.5	60.5
ndhG-ndhI_p5	CATGTATCGGTCCAGGCAAA	CTCCCCGTTGTTGATTGGAA	50.0	50.0	58.0	58.1	648-650 bp	0.5	60.3