primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	ndhJ-ndhK	1	GAAGACAAACGACCCTGCAT	ACAAAAGTTCAGTATCTTCCTACGA	20	25	50.0	36.0	58.5	58.0	0.0	0.0	5.486583	52777	52889	163-218	1.000000	48.9
ndhJ-ndhK_p2	ndhJ-ndhK	2	CCAAGAAGACAAACGACCCT	ACAAAAGTTCAGTATCTTCCTACGA	20	25	50.0	36.0	57.7	58.0	0.0	0.0	5.266458	52777	52889	167-222	1.000000	48.8
ndhJ-ndhK_p3	ndhJ-ndhK	3	AAGAAGACAAACGACCCTGC	ACAAAAGTTCAGTATCTTCCTACGA	20	25	50.0	36.0	58.4	58.0	0.0	0.0	5.416010	52777	52889	165-220	1.000000	48.8
ndhJ-ndhK_p4	ndhJ-ndhK	4	CCAAGAAGACAAACGACCCT	ACAAAAGTTCAGTATCTTCCTACG	20	24	50.0	37.5	57.7	56.7	0.0	0.0	5.525804	52777	52889	167-222	1.000000	48.8
ndhJ-ndhK_p5	ndhJ-ndhK	5	AGCCAAGAAGACAAACGACC	ACAAAAGTTCAGTATCTTCCTACGA	20	25	50.0	36.0	58.4	58.0	0.0	0.0	5.416010	52777	52889	169-224	1.000000	48.7
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	CGATGTGATAGGGCCCATTT	CTCTCAGACGAGCTAGGACA	20	20	50.0	55.0	57.7	58.0	27.6	0.0	0.307991	55695	55859	334-356	1.000000	82.0
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	CGATGTGATAGGGCCCATTT	CGAGCTAGGACACGAGTAGA	20	20	50.0	55.0	57.7	58.1	27.6	0.0	0.339454	55695	55859	326-348	1.000000	81.8
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	CGATGTGATAGGGCCCATTT	AAAGCTCAGGGCAAAAGACA	20	20	50.0	45.0	57.7	57.9	27.6	0.0	0.348510	55695	55859	374-396	1.000000	81.2
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	CGATGTGATAGGGCCCATTT	AAGCTCAGGGCAAAAGACAA	20	20	50.0	45.0	57.7	57.9	27.6	0.0	0.348510	55695	55859	373-395	1.000000	81.2
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	CGATGTGATAGGGCCCATTT	AGCTCAGGGCAAAAGACAAA	20	20	50.0	45.0	57.7	57.9	27.6	0.0	0.348510	55695	55859	372-394	1.000000	81.2
ndhF_p1	ndhF	1	ACAGGAACAAGAGGGATCCA	TGGATCAACTTGTAGGCTGTC	20	21	50.0	47.6	58.0	57.7	15.2	0.0	1.379375	112930	115176	2989-2989	0.250000	39.0
ndhF_p2	ndhF	2	AAGAGGGATCCACCGAAGAA	TGGATCAACTTGTAGGCTGTC	20	21	50.0	47.6	58.0	57.7	15.2	0.0	1.381961	112930	115176	2981-2981	0.250000	38.9
ndhF_p3	ndhF	3	ACAGGAACAAGAGGGATCCA	ACTGGATCAACTTGTAGGCTG	20	21	50.0	47.6	58.0	57.7	15.2	0.0	1.385607	112930	115176	2991-2991	0.250000	38.9
ndhF_p4	ndhF	4	ACAGGAACAAGAGGGATCCA	CTGGATCAACTTGTAGGCTGT	20	21	50.0	47.6	58.0	57.7	15.2	0.0	1.385607	112930	115176	2990-2990	0.250000	38.9
ndhF_p5	ndhF	5	AAGAGGGATCCACCGAAGAA	CTGGATCAACTTGTAGGCTGT	20	21	50.0	47.6	58.0	57.7	15.2	0.0	1.388193	112930	115176	2982-2982	0.250000	38.9
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCCGTCGGAAAAAGTA	AAAAAGCTTTCAACGCTGCC	20	20	45.0	45.0	58.0	58.4	17.5	13.1	0.478359	115177	115829	738-849	0.500000	64.2
ndhF-rpl32_p2	ndhF-rpl32	2	CCCCACGCGATTAGTTACAA	AAAAAGCTTTCAACGCTGCC	20	20	50.0	45.0	58.0	58.4	15.9	13.1	0.442124	115177	115829	1194-1305	0.500000	63.3
ndhF-rpl32_p3	ndhF-rpl32	3	CCCCACGCGATTAGTTACAA	AGTCATCCGCTCAAAACAGT	20	20	50.0	45.0	58.0	57.4	15.9	0.0	0.563767	115177	115829	1479-1608	0.500000	62.0
ndhF-rpl32_p4	ndhF-rpl32	4	CCCCACGCGATTAGTTACAA	TGTTTTATTGGGGCGATGGG	20	20	50.0	50.0	58.0	58.5	15.9	0.0	0.533228	115177	115829	1815-1815	0.250000	45.7
ndhF-rpl32_p5	ndhF-rpl32	5	TGTTGCCGTCGGAAAAAGTA	TGTTTTATTGGGGCGATGGG	20	20	45.0	50.0	58.0	58.5	17.5	0.0	0.569463	115177	115829	1359-1359	0.250000	45.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.4	58.0	14.0	0.0	0.437017	115992	117068	1166-1191	0.500000	60.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.4	58.0	14.0	0.0	0.438388	115992	117068	1167-1192	0.500000	60.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGCAGCGTTGAAAGCTTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.4	57.9	14.0	0.0	0.553464	115992	117068	1176-1201	0.500000	59.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGCAGCGTTGAAAGCTTTTT	AAAATAGTACTTGGCGCGGT	20	20	45.0	45.0	58.4	57.9	14.0	8.8	0.532724	115992	117068	1644-1669	0.500000	59.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGCAGCGTTGAAAGCTTTTT	AAATAGTACTTGGCGCGGTT	20	20	45.0	45.0	58.4	57.9	14.0	8.8	0.532724	115992	117068	1643-1668	0.500000	59.0
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	GTGAAATCGGTAGACACGCT	AAAATAGTACTTGGCGCGGT	20	20	50.0	45.0	58.0	57.9	0.0	8.8	0.111367	117149	117246	498-498	0.500000	60.1
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	GTGAAATCGGTAGACACGCT	AAATAGTACTTGGCGCGGTT	20	20	50.0	45.0	58.0	57.9	0.0	8.8	0.111367	117149	117246	497-497	0.500000	60.1
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	TGAAATCGGTAGACACGCTG	AAAATAGTACTTGGCGCGGT	20	20	50.0	45.0	58.0	57.9	0.0	8.8	0.112738	117149	117246	497-497	0.500000	60.1
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	TGAAATCGGTAGACACGCTG	AAATAGTACTTGGCGCGGTT	20	20	50.0	45.0	58.0	57.9	0.0	8.8	0.112738	117149	117246	496-496	0.500000	60.1
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	GAGAAAGCCGCTATGGTGAA	AAATAGTACTTGGCGCGGTT	20	20	50.0	45.0	58.0	57.9	0.0	8.8	0.127713	117149	117246	512-512	0.500000	59.9
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	8.5	0.040512	117247	118218	1758-1767	0.500000	61.5
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	8.5	0.041882	117247	118218	1757-1766	0.500000	61.5
ccsA_p3	ccsA	3	GAGAAAGCCGCTATGGTGAA	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	8.5	0.056857	117247	118218	1773-1782	0.500000	61.4
ccsA_p4	ccsA	4	GTGAAATCGGTAGACACGCT	CATGTTTGTGTTCGTCAGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044387	117247	118218	1177-1177	0.500000	60.7
ccsA_p5	ccsA	5	TGAAATCGGTAGACACGCTG	CATGTTTGTGTTCGTCAGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045757	117247	118218	1176-1176	0.500000	60.7
ndhD_p1	ndhD	1	GCCTGACGAACACAAACATG	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.055300	118473	120062	1918-1932	0.500000	61.0
ndhD_p2	ndhD	2	GCCTGACGAACACAAACATG	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067453	118473	120062	1924-1938	0.500000	60.9
ndhD_p3	ndhD	3	GCCTGACGAACACAAACATG	CCGACGGATTTCTTGAGTGT	20	20	50.0	50.0	58.0	57.9	0.0	8.6	0.116843	118473	120062	1958-1972	0.500000	60.5
ndhD_p4	ndhD	4	GAATTCACGAAAGGGCCTGA	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.2	58.0	6.9	0.0	0.197527	118473	120062	1932-1946	0.500000	59.9
ndhD_p5	ndhD	5	GCCTGACGAACACAAACATG	TTCGGCTCCAAGAACAGAAG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.206389	118473	120062	2013-2013	0.250000	48.3
psaC-ndhE_p1	psaC-ndhE	1	AGAAAAGTAAGCAACCCGCA	TCTTAGCTGCTTTGATCGGC	20	20	45.0	50.0	58.0	58.3	0.0	7.8	0.367688	120367	120613	1276-1282	0.500000	58.2
psaC-ndhE_p2	psaC-ndhE	2	AGAAAAGTAAGCAACCCGCA	GGATTGATCACGAGCCGAAA	20	20	45.0	50.0	58.0	58.3	0.0	0.0	0.372353	120367	120613	954-960	0.500000	57.9
psaC-ndhE_p3	psaC-ndhE	3	GCTTCGAGTTGTTTCATGCC	TCTTAGCTGCTTTGATCGGC	20	20	50.0	50.0	58.0	58.3	0.0	7.8	0.374244	120367	120613	1046-1047	0.500000	57.8
psaC-ndhE_p4	psaC-ndhE	4	ACCCATGCTTCGAGTTGTTT	TCTTAGCTGCTTTGATCGGC	20	20	45.0	50.0	58.0	58.3	0.0	7.8	0.362091	120367	120613	1052-1052	0.250000	47.1
psaC-ndhE_p5	psaC-ndhE	5	ACCCATGCTTCGAGTTGTTT	GGATTGATCACGAGCCGAAA	20	20	45.0	50.0	58.0	58.3	0.0	0.0	0.366756	120367	120613	730-730	0.250000	47.1
ndhG-ndhI_p1	ndhG-ndhI	1	CATGTATCGGTCCAGGCAAA	AACAAACAGTACGAGCTGCA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.088853	121672	122032	842-844	0.500000	60.5
ndhG-ndhI_p2	ndhG-ndhI	2	CATGTATCGGTCCAGGCAAA	ACAAACAGTACGAGCTGCAA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.088853	121672	122032	841-843	0.500000	60.5
ndhG-ndhI_p3	ndhG-ndhI	3	CAAGAACCAGTCCCAACGAA	AACAAACAGTACGAGCTGCA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.090102	121672	122032	947-949	0.500000	60.5
ndhG-ndhI_p4	ndhG-ndhI	4	CAAGAACCAGTCCCAACGAA	ACAAACAGTACGAGCTGCAA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.090102	121672	122032	946-948	0.500000	60.5
ndhG-ndhI_p5	ndhG-ndhI	5	CATGTATCGGTCCAGGCAAA	CTCCCCGTTGTTGATTGGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.132671	121672	122032	648-650	0.500000	60.3
ndhI_p1	ndhI	1	CATGTATCGGTCCAGGCAAA	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.087492	122033	122527	1618-1620	0.500000	60.0
ndhI_p2	ndhI	2	CAAGAACCAGTCCCAACGAA	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088741	122033	122527	1723-1725	0.500000	60.0
ndhI_p3	ndhI	3	CGGTCCAGGCAAATCCATTA	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.101869	122033	122527	1611-1613	0.500000	59.9
ndhI_p4	ndhI	4	CATGTATCGGTCCAGGCAAA	TGGTTTTTGGGGGTGGAATT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.235291	122033	122527	1449-1451	0.500000	58.9
ndhI_p5	ndhI	5	CAAGAACCAGTCCCAACGAA	TGGTTTTTGGGGGTGGAATT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.236540	122033	122527	1554-1556	0.500000	58.9
ndhA_p1	ndhA	1	TGCAGCTCGTACTGTTTGTT	CAGACTTGGTGAGATGACGG	20	20	45.0	55.0	58.1	58.0	8.8	0.0	0.053708	122608	124829	2754-2757	0.500000	60.1
ndhA_p2	ndhA	2	TTGCAGCTCGTACTGTTTGT	CAGACTTGGTGAGATGACGG	20	20	45.0	55.0	58.1	58.0	8.8	0.0	0.053708	122608	124829	2755-2758	0.500000	60.1
ndhA_p3	ndhA	3	TGCAGCTCGTACTGTTTGTT	ATTGGGGGTTATCAGGACCA	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.057497	122608	124829	2896-2899	0.500000	60.1
ndhA_p4	ndhA	4	TGCAGCTCGTACTGTTTGTT	TGGGGGTTATCAGGACCAAT	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.057497	122608	124829	2894-2897	0.500000	60.1
ndhA_p5	ndhA	5	TTGCAGCTCGTACTGTTTGT	TGGGGGTTATCAGGACCAAT	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.057497	122608	124829	2895-2898	0.500000	60.1
ndhH_p1	ndhH	1	AGGTCTCAACGAATCACACG	TGGGAAAACGCCAACGATTA	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.172448	124831	126012	1963-1963	0.500000	59.0
ndhH_p2	ndhH	2	GGAGCAACACTTGAAATGGC	TGGGAAAACGCCAACGATTA	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.176717	124831	126012	1816-1816	0.500000	59.0
ndhH_p3	ndhH	3	TAAGAAGTCCGATGGGAGCA	TGGGAAAACGCCAACGATTA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.245814	124831	126012	1830-1830	0.500000	58.4
ndhH_p4	ndhH	4	AGGTCTCAACGAATCACACG	GGGAAAACGCCAACGATTAC	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.317862	124831	126012	1962-1962	0.500000	57.8
ndhH_p5	ndhH	5	GGAGCAACACTTGAAATGGC	GGGAAAACGCCAACGATTAC	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.322131	124831	126012	1815-1815	0.500000	57.8
rps15-ycf1_p1	rps15-ycf1	1	TAATCGTTGGCGTTTTCCCA	AAAGCTCTTTCTTTGGCCCA	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.193066	126390	126800	713-733	0.500000	62.1
rps15-ycf1_p2	rps15-ycf1	2	TAATCGTTGGCGTTTTCCCA	AAGCTCTTTCTTTGGCCCAA	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.193066	126390	126800	712-732	0.500000	62.1
rps15-ycf1_p3	rps15-ycf1	3	ATGAGTCGAAGAACACCGTG	AAAGCTCTTTCTTTGGCCCA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.152603	126390	126800	987-1007	0.500000	61.4
rps15-ycf1_p4	rps15-ycf1	4	ATGAGTCGAAGAACACCGTG	AAGCTCTTTCTTTGGCCCAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.152603	126390	126800	986-1006	0.500000	61.4
rps15-ycf1_p5	rps15-ycf1	5	CGTTTCTTGTAGCTGGTCCA	AAGCTCTTTCTTTGGCCCAA	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.250428	126390	126800	925-945	0.500000	60.8
ycf1_p1	ycf1	1	ACCCCGTAACCAGTTTTTCC	CCTTTTGGTTCTCCCCGAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024821	128985	129104	1053-1059	0.500000	61.4
ycf1_p2	ycf1	2	CCCCGTAACCAGTTTTTCCA	CCTTTTGGTTCTCCCCGAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026228	128985	129104	1052-1058	0.500000	61.4
ycf1_p3	ycf1	3	ACCCCGTAACCAGTTTTTCC	GGTTCTCCCCGAAAACAACT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.037471	128985	129104	1047-1053	0.500000	61.3
ycf1_p4	ycf1	4	ATGCTTGCGACAAGTACGAT	CCTTTTGGTTCTCCCCGAAA	20	20	45.0	50.0	58.0	58.0	17.0	0.0	0.020439	128985	129104	841-841	0.500000	60.9
ycf1_p5	ycf1	5	ATGCTTGCGACAAGTACGAT	GGTTCTCCCCGAAAACAACT	20	20	45.0	50.0	58.0	58.0	17.0	0.0	0.033089	128985	129104	835-835	0.500000	60.8