Hotspot

rps16

LSC · 4498-5545 · 1048 bp

Back to Thinopyrum

Pi 0.00172

MarkerSeek score 60.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1048
variable_sites: 4
pi: 0.00172
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGGCACGATATAAATCCAAATAGG	ACCACTTTGCAATGAAAAGAGG	37.5	40.9	56.9	57.7	1198-1199 bp	1.0	40.3
rps16_p2	TGGCACGATATAAATCCAAATAGG	CCACTTTGCAATGAAAAGAGGA	37.5	40.9	56.9	57.4	1197-1198 bp	1.0	40.3
rps16_p3	TGGCACGATATAAATCCAAA	ACCACTTTGCAATGAAAAGAGG	35.0	40.9	52.6	57.7	1198-1199 bp	1.0	40.3
rps16_p4	TTGGCACGATATAAATCCAA	ACCACTTTGCAATGAAAAGAGG	35.0	40.9	52.6	57.7	1199-1200 bp	1.0	40.3
rps16_p5	TTGGCACGATATAAATCCAAA	ACCACTTTGCAATGAAAAGAGG	33.3	40.9	53.6	57.7	1199-1200 bp	1.0	40.3