Hotspot

rpl22

LSC · 80142-80591 · 450 bp

Back to Thinopyrum

Pi 0.00422

MarkerSeek score 37.40

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 450
variable_sites: 4
pi: 0.00422
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CGCGTGCAATTTCTTTTCCT	CAAATTGGGGGAATTCGTGC	45.0	50.0	57.9	58.0	1254-1273 bp	1.0	80.0
rpl22_p2	CGCGTGCAATTTCTTTTCCT	GCAAATTGGGGGAATTCGTG	45.0	50.0	57.9	58.0	1255-1274 bp	1.0	80.0
rpl22_p3	GTGCGAACCAAAAGGAATGG	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.9	58.0	738-757 bp	1.0	80.0
rpl22_p4	GTGCGAACCAAAAGGAATGG	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.9	58.0	739-758 bp	1.0	80.0
rpl22_p5	CGCGTGCAATTTCTTTTCCT	GTTATCCTGCGCTTGGAAGA	45.0	50.0	57.9	58.0	1795-1814 bp	1.0	79.8