Hotspot

rpl32-trnL-UAG

SSC · 104818-105520 · 703 bp

Back to Thinopyrum

Pi 0.00398
MarkerSeek score 47.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
703
variable_sites
7
pi
0.00398
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTCCAGCGGTAATGAGCATC TTCGAACCGAGATGCTTGAG 50.0 50.0 58.0 58.0 839-841 bp 1.0 80.6
rpl32-trnL-UAG_p2 TTCCAGCGGTAATGAGCATC GGATTCGAACCGAGATGCTT 50.0 50.0 58.0 58.1 842-844 bp 1.0 80.1
rpl32-trnL-UAG_p3 TTCCAGCGGTAATGAGCATC GATCCGCACAAAAGAGTTGC 50.0 50.0 58.0 58.0 1385-1387 bp 1.0 80.0
rpl32-trnL-UAG_p4 AAGGGTTTTTCTGGGCAACA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 811-813 bp 1.0 79.9
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 810-812 bp 1.0 79.9