Hotspot

ndhF-rpl32

SSC · 103726-104628 · 903 bp

Back to Thinopyrum

Pi 0.00713

MarkerSeek score 62.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 903
variable_sites: 15
pi: 0.00713
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TTGACATTGAAGTACGTTTTT	55.0	28.6	58.1	52.2	987-996 bp	1.0	41.7
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TTTGACATTGAAGTACGTTTT	55.0	28.6	58.1	52.2	988-997 bp	1.0	41.7
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTTTGACATTGAAGTACGTTT	55.0	28.6	58.1	52.2	989-998 bp	1.0	41.7
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATTGAAGTACGTT	55.0	28.6	58.1	52.2	990-999 bp	1.0	41.7
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCT	55.0	29.2	58.1	55.3	1014-1023 bp	1.0	41.6