Hotspot

rpl20-rps12

LSC · 66817-67510 · 694 bp

Back to Thinopyrum

Pi 0.00259

MarkerSeek score 60.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 694
variable_sites: 4
pi: 0.00259
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGTCGTC	GAGGAACATGTGCTAGGGTG	45.0	55.0	58.0	58.0	771 bp	1.0	80.1
rpl20-rps12_p2	ACGCATTTTTGTTCGTCGTC	GTCGAGGAACATGTGCTAGG	45.0	55.0	58.0	58.1	774 bp	1.0	79.7
rpl20-rps12_p3	GTTTGAGGCAAATGAACGCA	GAGGAACATGTGCTAGGGTG	45.0	55.0	57.9	58.0	786 bp	1.0	79.1
rpl20-rps12_p4	GTTTGAGGCAAATGAACGCA	GTCGAGGAACATGTGCTAGG	45.0	55.0	57.9	58.1	789 bp	1.0	78.8
rpl20-rps12_p5	ACGCATTTTTGTTCGTCGTC	TAGAAACGCAAGACAGCCAA	45.0	45.0	58.0	57.8	839 bp	1.0	78.3