Hotspot

rbcL-rpl23

LSC · 55850-56142 · 293 bp

Back to Thinopyrum

Pi 0.0109

MarkerSeek score 50.90

Primer available yes

Label rbcL-rpl23

Feature Metadata

label_name: rbcL-rpl23
feature_type: igs
region: LSC
length_bp: 293
variable_sites: 6
pi: 0.0109
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-rpl23_p1	CCGAAATCTTTGGGGACGAT	GACTCACGATTACGCCTGTT	50.0	50.0	58.0	58.0	922-948 bp	1.0	83.1
rbcL-rpl23_p2	CCGAAATCTTTGGGGACGAT	GTTGACTCACGATTACGCCT	50.0	50.0	58.0	58.0	925-951 bp	1.0	83.1
rbcL-rpl23_p3	CCGAAATCTTTGGGGACGAT	TGTCCAACCCCAAATCAAGG	50.0	50.0	58.0	58.0	1084-1110 bp	1.0	82.7
rbcL-rpl23_p4	CTTAACTGGGGGATTCACCG	GACTCACGATTACGCCTGTT	55.0	50.0	58.0	58.0	1287-1313 bp	1.0	82.1
rbcL-rpl23_p5	CTTAACTGGGGGATTCACCG	GTTGACTCACGATTACGCCT	55.0	50.0	58.0	58.0	1290-1316 bp	1.0	82.1