Hotspot

ycf3-trnS-GGA

LSC · 43816-44430 · 615 bp

Back to Thinopyrum

Pi 0.00325

MarkerSeek score 61.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 615
variable_sites: 5
pi: 0.00325
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	798 bp	1.0	78.8
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.1	57.8	748 bp	1.0	78.5
ycf3-trnS-GGA_p3	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	799 bp	1.0	77.0
ycf3-trnS-GGA_p4	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	800 bp	1.0	77.0
ycf3-trnS-GGA_p5	TTCTCCTGAGGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.3	57.8	749 bp	1.0	76.7