Hotspot

rpl32-ndhF

SSC · 131244-132483 · 1240 bp

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Pi 0.0376

MarkerSeek score 68.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1240
variable_sites: 46
pi: 0.0376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TGGGCGATGGGGAAAATAAG	TGTTGCCGTCGGAAAAAGTA	50.0	45.0	57.9	58.0	1613-1620 bp	1.0	82.2
rpl32-ndhF_p2	TGGGCGATGGGGAAAATAAG	GCCCACATACGACGAAGATT	50.0	50.0	57.9	58.1	1636-1643 bp	1.0	82.1
rpl32-ndhF_p3	AAATGGGCGATGGGGAAAAT	TGTTGCCGTCGGAAAAAGTA	45.0	45.0	58.1	58.0	1616-1623 bp	1.0	81.8
rpl32-ndhF_p4	AAATGGGCGATGGGGAAAAT	GCCCACATACGACGAAGATT	45.0	50.0	58.1	58.1	1639-1646 bp	1.0	81.6
rpl32-ndhF_p5	TGGGCGATGGGGAAAATAAG	AAAAGCCCACATACGACGAA	50.0	45.0	57.9	57.8	1640-1647 bp	1.0	81.2