Hotspot

trnL-UAG-rpl32

SSC · 129954-131078 · 1125 bp

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Pi 0.0286
MarkerSeek score 66.10
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
1125
variable_sites
32
pi
0.0286
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAGAAAGGATATTGGGCGGC 50.0 50.0 58.0 58.3 1256-1291 bp 1.0 79.8
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAGAAAGGATATTGGGCGGC 50.0 50.0 58.0 58.3 1257-1292 bp 1.0 79.8
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA AAGAAAGGATATTGGGCGGC 50.0 50.0 57.9 58.3 1266-1301 bp 1.0 78.9
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC AAGAAAGGATATTGGGCGGC 55.0 50.0 57.8 58.3 1265-1300 bp 1.0 78.3
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC AAGAAAGGATATTGGGCGGC 55.0 50.0 57.8 58.3 1264-1299 bp 1.0 78.3