Hotspot

rpl22-rps19

LSC · 88606-88691 · 86 bp

Back to Thespesia

Pi 0.0779

MarkerSeek score 72.60

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 86
variable_sites: 6
pi: 0.0779
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ATTCCCTCGACACCAGAAGA	AACTGATTGCAAAGGAGGGG	50.0	50.0	58.1	58.1	1468 bp	1.0	80.7
rpl22-rps19_p2	TTCCCTCGACACCAGAAGAT	AACTGATTGCAAAGGAGGGG	50.0	50.0	58.1	58.1	1467 bp	1.0	80.7
rpl22-rps19_p3	ATACGTGCAATTCCCTCGAC	AACTGATTGCAAAGGAGGGG	50.0	50.0	58.1	58.1	1477 bp	1.0	80.7
rpl22-rps19_p4	ATTCCCTCGACACCAGAAGA	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.1	58.1	1349 bp	1.0	80.7
rpl22-rps19_p5	TTCCCTCGACACCAGAAGAT	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.1	58.1	1348 bp	1.0	80.7