Hotspot

psbH-petB

LSC · 79306-79433 · 128 bp

Back to Thespesia

Pi 0.0391

MarkerSeek score 75.00

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 128
variable_sites: 5
pi: 0.0391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	CGCAATGGCTTTATTTGCGA	CGATCAAGATGACAGACCCA	45.0	50.0	58.1	57.0	283 bp	1.0	72.9
psbH-petB_p2	TCGCAATGGCTTTATTTGCG	CGATCAAGATGACAGACCCA	45.0	50.0	58.1	57.0	284 bp	1.0	72.9
psbH-petB_p3	CGCAATGGCTTTATTTGCGA	TCCGTGCTCCAGATACTAGA	45.0	50.0	58.1	57.0	331 bp	1.0	72.3
psbH-petB_p4	TCGCAATGGCTTTATTTGCG	TCCGTGCTCCAGATACTAGA	45.0	50.0	58.1	57.0	332 bp	1.0	72.3
psbH-petB_p5	AACGACTCCTTTGATGGGTG	CGATCAAGATGACAGACCCA	50.0	50.0	57.8	57.0	304 bp	1.0	72.1